Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.

نویسندگان

  • S A Brown
  • K Sugimoto
  • G G Smith
  • F B Garry
چکیده

Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 32 4  شماره 

صفحات  -

تاریخ انتشار 1988